Products
SH2B1 antibody
Category:
- Catalogue No.:
- FNab07818
- Alias:
- SH2B adapter protein 1|Pro-rich antibody, PH and SH2 domain-containing signaling mediator (PSM)|SH2 domain-containing protein 1B|SH2B1|KIAA1299|SH2B antibody
- Gene Name:
- SH2B1
- UniProt ID:
- Q9NRF2
- Product Name
- SH2B1 antibody
- Catalogue No.
- FNab07818
- Gene Name
- SH2B1
- UniProt ID
- Q9NRF2
- Alias
- SH2B adapter protein 1|Pro-rich antibody, PH and SH2 domain-containing signaling mediator (PSM)|SH2 domain-containing protein 1B|SH2B1|KIAA1299|SH2B antibody
- Isotype
- IgG
- Form
- liquid
- Purification
- Immunogen affinity purified
- Immunogen
- SH2B adaptor protein 1
- Recommended dilution
- WB: 1:200-1:2000; IP: 1:200-1:2000; IHC: 1:20-1:200; IF: 1:20-1:200
- Purity
- ≥95% as determined by SDS-PAGE
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
- Background
- Adapter protein for several members of the tyrosine kinase receptor family. Involved in multiple signaling pathways mediated by Janus kinase(JAK) and receptor tyrosine kinases, including the receptors of insulin(INS), insulin-like growth factor I(IGF1), nerve growth factor(NGF), brain-derived neurotrophic factor(BDNF), glial cell line-derived neurotrophic factor(GDNF), platelet-derived growth factor(PDGF) and fibroblast growth factors(FGFs). In growth hormone(GH) signaling, autophosphorylated('Tyr-813') JAK2 recruits SH2B1, which in turn is phosphorylated by JAK2 on tyrosine residues. These phosphotyrosines form potential binding sites for other signaling proteins. GH also promotes serine/threonine phosphorylation of SH2B1 and these phosphorylated residues may serve to recruit other proteins to the GHR-JAK2-SH2B1 complexes, such as RAC1. In leptin(LEP) signaling, binds to and potentiates the activation of JAK2 by globally enhancing downstream pathways. In response to leptin, binds simultaneously to both, JAK2 and IRS1 or IRS2, thus mediating formation of a complex of JAK2, SH2B1 and IRS1 or IRS2. Mediates tyrosine phosphorylation of IRS1 and IRS2, resulting in activation of the PI 3-kinase pathway. Acts as positive regulator of NGF-mediated activation of the Akt/Forkhead pathway; prolongs NGF-induced phosphorylation of AKT1 on 'Ser-473' and AKT1 enzymatic activity. Enhances the kinase activity of the cytokine receptor-associated tyrosine kinase JAK2 and of other receptor tyrosine kinases, such as FGFR3 and NTRK1. For JAK2, the mechanism seems to involve dimerization of both, SH2B1 and JAK2. Enhances RET phosphorylation and kinase activity. Isoforms seem to be differentially involved in IGF-I and PDGF-induced mitogenesis(By similarity).
Figures for this product are not available in this catalogue.
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
- Dispatch Time
- About 3 working days
Ordering & Delivery
How do I place an order?
Use the Inquiry button on this page or write to tekintolga89@gmail.com. Include the catalogue number and the quantity you need.
What is the dispatch time?
In-stock items are dispatched within 1-3 business days. Items that need to be produced take longer; we confirm the date when you order.
Do you ship outside Türkiye?
Shipping is arranged case by case. Contact us with the destination and we will quote carriage and lead time.
Storage & Handling
How should an unopened kit be stored?
At 2-8 °C unless the datasheet says otherwise. Do not freeze a kit that is not marked for freezing.
How long is an opened kit stable?
It depends on the component. The Kit Components table on this page lists the storage condition for each item after opening.
What should I do if the shipment arrives warm?
Photograph the package and contact us the same day. Do not use the kit until we have confirmed it is still within specification.
Assay Questions
Which sample types can I use?
The Sample Type row in the specifications above lists the validated matrices for this product. Other matrices need validation on your side.
My background is too high. What should I check?
The usual causes are insufficient washing, contaminated buffers, or too much detection antibody. Our Protocol pages cover the full troubleshooting sequence.
Can I run fewer wells than the kit provides?
Yes. Dismountable microplates let you use part of a plate and reseal the rest with the desiccant supplied.
Technical Support
Something did not work. What do you need from me?
Send the catalogue number, the lot, your protocol deviations and the raw OD values. That is usually enough to identify the cause.
Do you provide a certificate of analysis?
Yes, per lot. Ask for it with the catalogue number and lot number.
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Product Inquiry
FNab07818 — SH2B1 antibody